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  • Title: Production of a Novel Extracellular Polysaccharide by a Bacillus Strain Isolated from Soil.
    Author: Kawai H, Isobe Y, Horibe M, Tokuda J, Tokuno I, Endo K, Kawai F.
    Journal: Biosci Biotechnol Biochem; 1992 Jan; 56(6):853-7. PubMed ID: 27280807.
    Abstract:
    A bacterium that was isolated from soil and identified as Bacillus circulans was found to produce a highly viscous extracellular polysaccharide when it was grown aerobically in a medium containing glucose as a sole source of carbon. The product was characterized by TLC and GC analyses as a novel heteropolysaccharide consisted of rhamnose, mannose, galactose, and mannuronic acid as sugar components. A maximal yield of polysaccharide reached about 2 g/liter by jar-fermentor culture at 30°C for 48 hr with a medium containing 1% glucose, 0.05% asparagine, 0.005% yeast extract, and small amounts of inorganic salts. Some culture conditions for the production of polysaccharide were investigated with flask culture; an optimal production was attained with a medium containing 0.1-1 % glucose and 0.01-0.05% asparagine, pH 7-8, at 30°C under aerobic conditions.
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