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  • Title: Fertilization and development of mouse eggs injected under the zona pellucida with single spermatozoa treated to induce the acrosome reaction.
    Author: Lacham O, Trounson A, Holden C, Mann J, Sathananthan H.
    Journal: Gamete Res; 1989 Jun; 23(2):233-43. PubMed ID: 2731906.
    Abstract:
    Mouse spermatozoa were capacitated and acrosome reacted by incubation for 30 min to 6 h in modified Tyrode's medium (T6) and in cGMP. The fertilization rates of eggs microinjected with a spermatozoon from samples incubated for 30 min, 2 h, and 6 h in T6 and in cGMP were 36%, 34%, 29%, and 43%, respectively. These rates were not correlated to the percentage of acrosome-reacted spermatozoa identified after these treatments. The lack of association could be explained by the selection of an increased proportion of acrosome-reacted spermatozoa actually used for microinjection, particularly in samples incubated for 30 min and 2 h in T6. Both acrosome-intact and acrosome-reacted spermatozoa were identified under the zona in the eggs which failed to fertilize. Fertilized eggs developed at very high rates to the blastocyst stage in vitro.
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