These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A further study of human seminal plasma lactate dehydrogenase-C4 (LDH-C4): kinetic properties of LDH-C4.
    Author: Uchijima Y, Yoshida K, Saito H.
    Journal: Hinyokika Kiyo; 1989 Mar; 35(3):457-63. PubMed ID: 2735254.
    Abstract:
    Lactate dehydrogenase-C4 (LDH-C4) from human seminal plasma was purified by 40-60% (NH4)2SO4 precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Enzyme kinetic studies of human seminal plasma LDH-C4 were performed with purified LDH -C4. The human LDH-C4 exhibits a much higher affinity for pyruvate and alpha-ketovalerate than lactate. Apparent Km values for pyruvate, alpha-ketovalerate and lactate were 7.7 X 10(-5) M, 1.3 X 10(-3) M and 6.7 X 10(-3) M, respectively. The human seminal plasma LDH-C4 was severely inhibited by increasing concentration of substrate, especially lactate. These kinetic properties were compared with those of previous reports of human spermatozoa.
    [Abstract] [Full Text] [Related] [New Search]