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  • Title: Regulation of idiotype expression. II. The phenotypic diversity of T15 idiotype-bearing antibody to phosphorylcholine in response to T-dependent and T-independent antigens.
    Author: Strickland FM, Cronkhite RI, Cerny J.
    Journal: Immunology; 1989 May; 67(1):8-15. PubMed ID: 2737696.
    Abstract:
    The idiotypic (Id) diversity of the immune response to phosphorylcholine (PC) was studied by immunization of mice with thymus-dependent (PC-keyhole limpet haemocyanin; PC-KLH) and thymus-independent (S. pneumoniae R36a; Pn) forms of the antigen. Mice with the BALB/c genetic background (BALB/c, C.B20, and BALB.B) were used because their response to PC is dominated by immunoglobulins encoded in VH-1 and V kappa 22 genes, which uniformly express the T15 idiotype. The actual repertoire of the antibody was determined by idiotypic markers (Id) defined with monoclonal antibodies designated AB1-2, B36-82, MaId5-4, and B24-44. Previous studies from our laboratory have shown that these Id are present on T15 (VS107-1/V kappa 22) immunoglobulins only, but that they differentiate between somatic variants of the antibody molecules. We have measured the serum concentrations of these four Id after primary (1 degree), secondary (2 degree), and tertiary (3 degree) immunization; all of the Id activity was associated with the PC-binding antibody, as shown by specific immunoadsorbents. However, the levels of the Id-bearing (Id+) antibody did not correlate with each other. After immunization with PC-KLH, the AB1-2+ antibody declined precipitously, whereas the levels of B24-44 and B36-82 remained steady. A similar pattern of Id heterogeneity was seen at the level of direct antibody-plaque-forming cells from the spleen, suggesting that the idiotopic (clonal) diversification occurred already during the early IgM response. A significant portion of anti-PC antibody after the 3 degrees PC-KLH immunization was negative for all four Id, implying that the late response to the antigen involved distinct, T15-negative clones.
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