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  • Title: Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody.
    Author: Matsubayashi M, Minoura C, Kimura S, Tani H, Furuya M, Lillehoj HS, Matsuda H, Takenaka S, Hatta T, Tsuji N, Sasai K.
    Journal: Parasitol Res; 2016 Nov; 115(11):4123-4128. PubMed ID: 27384177.
    Abstract:
    In the poultry industry, Eimeria spp. is one of the important pathogens which cause significant economic losses. We have previously generated a chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for an antigen located in the apical cytoskeleton of Eimeria acervulina and with cross-reactive among Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp. Furthermore, the protein of Cryptosporidium parvum recognized by the 6D-12-G10 has been identified as elongation factor-1α (EF-1α). In the present study, to identify the target molecule of E. acervulina by the mAb, we performed two-dimensional Western blotting analysis. Finally, we found two positive molecules which are identified as EF-1α and a related protein. Our previous finding using C. parvum and the results in this study suggest that EF-1α could be associated with the invasion facilitated by the cytoskeleton at the apical region of zoites.
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