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  • Title: Cryopreservation of in vitro shoot tips of strawberry by the vitrification method - establishment of a duplicate collection of Fragaria germplasm.
    Author: Höfer M.
    Journal: Cryo Letters; 2016; 37(3):163-72. PubMed ID: 27393953.
    Abstract:
    BACKGROUND: The National German Strawberry Genebank, which includes 183 cultivars and 270 accessions of wild Fragaria species, is maintained in a field collection of the Fruit Genebank at Dresden-Pillnitz. OBJECTIVE: A duplicate collection stored in liquid nitrogen would provide a higher level of security for these irreplaceable genetic resources. MATERIALS AND METHODS: Four distinct cryopreservation protocols were tested earlier and the best method, a PVS2 vitrification method with a 14-day alternating-temperature cold acclimation, was modified for further genotype screening. RESULTS: A comprehensive genotype spectrum was tested by screening 107 Fragaria ×ananassa cultivars and 20 Fragaria wild species (51 accessions). The average recovery for the cultivars using the optimized medium was 89.55 % and for the wild species 85.50 %. CONCLUSION: These results indicate that the method could be used to establish a backup collection in liquid nitrogen. Meristem cultures will be established and the collection will be cryopreserved using this vitrification technique to provide cost effective long-term storage.
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