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  • Title: Direct PCR-Ribotyping of Clostridium difficile.
    Author: Janezic S.
    Journal: Methods Mol Biol; 2016; 1476():15-21. PubMed ID: 27507330.
    Abstract:
    PCR-ribotyping, a method based on heterogeneity of ribosomal intergenic spacer region, is the preferred method for genotyping of Clostridium difficile. Standardly used procedure for PCR-ribotyping is culturing of C. difficile from fecal samples and subsequent typing. In this chapter, we describe a modified PCR-ribotyping method for direct detection of PCR-ribotypes directly in total stool DNA extract, without prior need to isolate C. difficile.
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