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  • Title: Two-step high-performance liquid chromatography method for the determination of myo-inositol and sorbitol.
    Author: Lauro PN, Craven PA, DeRubertis FR.
    Journal: Anal Biochem; 1989 May 01; 178(2):331-5. PubMed ID: 2751095.
    Abstract:
    A simple two-step HPLC method for the separation and quantitation of myo-inositol and sorbitol in extracts of glomeruli from rat kidneys is described. The limit of detection is 2 ng. The procedure involves fractionation of the sugar alcohols on a Waters Sugar Pak column, preparation of the p-nitrobenzoate derivatives, and further purification with quantitation by absorbance at 254 nm using a Waters mu Porasil column. The applicability of the procedure to determination of sorbitol and myo-inositol in biological samples was demonstrated by the finding of marked alterations in sorbitol and myo-inositol content of glomeruli isolated from diabetic compared to that from normal rat kidneys.
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