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  • Title: Confirmation of a ping-pong mechanism for S-adenosyl-L-methionine:magnesium protoporphyrin methyltransferase of etiolated wheat by an exchange reaction.
    Author: Yee WC, Eglsaer SJ, Richards WR.
    Journal: Biochem Biophys Res Commun; 1989 Jul 14; 162(1):483-90. PubMed ID: 2751667.
    Abstract:
    An exchange reaction between unlabeled S-adenosyl-L-methionine and radiolabeled S-adenosyl-L-homocysteine has been used to confirm the occurrence of a ping-pong mechanism in S-adenosyl-L-methionine:magnesium protoporphyrin methyltransferase of etiolated wheat. The enzyme, S-adenosyl-L-homocysteine hydrolase, has been used to prepare radiolabeled S-adenosyl-L-homocysteine from labeled adenosine and DL-homocysteine. The exchange reaction was accomplished with a methyltransferase preparation purified by affinity chromatography on hemin-linked Sepharose 4B, and radioactivity was exchanged into unlabeled S-adenosyl-L-methionine to an extent of 70% of the theoretical maximum value.
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