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  • Title: The polyene antibiotic amphotericin B acts as a Ca2+ ionophore in sterol-containing liposomes.
    Author: Ramos H, Attias de Murciano A, Cohen BE, Bolard J.
    Journal: Biochim Biophys Acta; 1989 Jul 10; 982(2):303-6. PubMed ID: 2752030.
    Abstract:
    Amphotericin B (AmB) was shown to induce a Ca2+ influx across ergosterol- and cholesterol-containing large unilamellar liposomes, by following spectrophotometrically the formation of the Arsenazo III-Ca2+ complex. At equivalent antibiotic concentrations the Ca2+ influx was much more extensive through ergosterol-containing membranes (almost 100% with 1 microM AmB, 160 microM lipid) than through cholesterol-containing membranes (below 0.5 microM the influx of Ca2+ was negligible). In the presence of ergosterol-containing membranes the initial rate of Ca2+ influx had the same linear dependence on the ratio antibiotic/lipid whatever the lipid concentration, which was not the case in cholesterol-containing membranes. These results suggest that the channels responsible for the AmB-induced Ca2+ permeability across cholesterol- and ergosterol-containing liposomes have different structures.
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