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  • Title: Metabolism of 24,25-dihydrolanosterol analogs by partially purified cytochrome P-450(14DM) from rat liver microsomes.
    Author: Sonoda Y, Sekigawa Y, Sato Y.
    Journal: Chem Pharm Bull (Tokyo); 1989 Mar; 37(3):718-22. PubMed ID: 2752483.
    Abstract:
    27-Nor-24,25-dihydrolanosterol (27-nor-DHL), 26,27-dinor-24,25- dihydrolanosterol (26,27-dinor-DHL), and 25,26,27-trinor-24,25-dihydrolanosterol (25,26,27-trinor-DHL), analogs of 24,25-dihydrolanosterol (DHL) which have no C-27 carbon, C-26,27 carbons and C-25,26,27 carbons, were converted to the corresponding 14-demethylated products using a reconstituted monooxygenase system from rat liver microsomes which contained cytochrome P-450(14DM) catalyzing lanosterol 14-demethylation and reduced nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P-450 reductase in the presence of NADPH and molecular oxygen. Each metabolite showed a relative retention time (RtR) of 0.72 with respect to each substrate in high-performance liquid chromatography (HPLC) on a reversed-phase column. Comparison of each gas chromatography-mass spectrum and RtR value with those of the metabolite of DHL, 4,4-dimethyl-5 alpha-cholesta-8,14-dien-3 beta-ol, indicated that the metabolites could be inferred to be 27-nor-4,4-dimethyl-5 alpha-cholesta-8,14-dien-3 beta-ol, 26,27-dinor-4,4- dimethyl-5 alpha-cholesta-8,14-dien-3 beta-ol, and 25,26,27-trinor-4,4-dimethyl-5 alpha-cholesta-8,14-dien-3 beta-ol. However, 24,25,26,27-tetranor- and 23,24,25,26,27-pentanor analogs of DHL and 20-iso-24,25-dihydrolanosterol were not metabolized by the reconstituted enzyme system.
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