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  • Title: Effect of phenobarbital on the expression of glutathione S-transferase isoenzymes in cultured rat hepatocytes.
    Author: Vandenberghe Y, Morel F, Foriers A, Ketterer B, Vercruysse A, Guillouzo A, Rogiers V.
    Journal: FEBS Lett; 1989 Jul 17; 251(1-2):59-64. PubMed ID: 2753164.
    Abstract:
    Cultured adult rat hepatocytes were treated daily with 3.2 mM phenobarbital (PB) in order to study its effect on the expression of cytosolic glutathione S-transferase isoenzymes. Glutathione S-transferase (GST) activities, using 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates, were increased when PB was present in the culture medium. After purification and separation of GST on glutathione Sepharose 6 B and reversed-phase HPLC, respectively, it was observed in vitro that PB caused an increase in the relative amounts of subunits 1, 3 and 7 compared to subunits 2 and 4. Using Northern blot technique, elevated levels of GST subunit 1/2 and 7 mRNA were measured, after addition of PB to the cultures.
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