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  • Title: Nonsulfated cholecystokinins in cerebral neurons.
    Author: Agersnap M, Zhang MD, Harkany T, Hökfelt T, Rehfeld JF.
    Journal: Neuropeptides; 2016 Dec; 60():37-44. PubMed ID: 27535680.
    Abstract:
    Cholecystokinin (CCK) is a widely expressed neuropeptide system originally discovered in the gut. Both cerebral and peripheral neurons as well as endocrine I-cells in the small intestine process proCCK to tyrosyl-O-sulfated and α-carboxyamidated peptides. Recently, we reported that gut endocrine I-cells also synthetize nonsulfated CCK in significant amounts. Accordingly, we have now examined whether porcine and rat cerebral tissues (four cortical regions, hypothalamus and cerebellum) also synthesize nonsulfated CCK. A new, specific radioimmunoassay showed that all brain samples from pigs (n=15) and rats (n=6) contained nonsulfated CCK. The highest concentrations were measured in the neocortex; 4.7±0.25pmol/g (7.4%) in the rat and 4.3±1.88pmol/g (2.3%) in the pig. Chromatography of porcine cortical extracts revealed that 96.4% of the CCK was O-sulfated CCK-8. A higher fraction of the larger peptides (CCK-58 and CCK-33) was nonsulfated in comparison with the shorter forms (CCK-22 and CCK-8), i.e., 8.1% and 4.3% versus 0.9% and 1.5%. Immunohistochemical analysis of the rat brain showed an overall similar distribution pattern in selected regions when comparing the antibody specific for nonsulfated CCK-8 with an antibody recognizing both sulfated and nonsulfated CCK. However, nonsulfated CCK immunoreactivity was stronger than that of sulfated CCK in cell bodies and weaker in nerve terminals. We conclude that only a small fraction of neuronal CCK is nonsulfated. The intracellular distribution of nonsulfated CCK in neurons suggests that they contribute only modestly to the CCK transmitter activity.
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