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  • Title: SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix.
    Author: Boysen M, Schlicksupp L, Dreher I, Loebbert R, Richter M.
    Journal: J Immunol Res; 2016; 2016():9096059. PubMed ID: 27556050.
    Abstract:
    Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles' heel, it is their potential immunogenicity. To better understand the impact of the size of immune complexes of mAbs on anti-drug antibody (ADA) dependent adverse reactions in Macaca fascicularis, we developed an efficient high-throughput size exclusion chromatography- (SEC-) based methodology that enables analysis of the size, size distribution, and ratio of free and ADA-complexed mAb in serum allowing for assessment of formation and clearance of circulating ADA-mAb immune complexes (CIC).
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