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  • Title: Intracellular pH during secretion in the perfused rabbit mandibular salivary gland measured by 31P NMR spectroscopy.
    Author: Steward MC, Seo Y, Case RM.
    Journal: Pflugers Arch; 1989 Jun; 414(2):200-7. PubMed ID: 2755773.
    Abstract:
    Intracellular pH (pHi) was measured in the isolated, perfused rabbit mandibular salivary gland by 31P NMR spectroscopy. In the unstimulated gland perfused with HCO3-/CO2-buffered Ringer's solution, pHi was 7.27 +/- 0.01. Continuous stimulation with acetylcholine elicited dose- and time-dependent changes in pHi. 10(-6) mol/l acetylcholine caused a brief intracellular acidosis (-0.19 +/- 0.06 pH units) followed by an increase in pHi to a more alkaline steady-state value (7.33 +/- 0.02). In the absence of perfusate HCO3- or in the presence of 10(-4) mol/l DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid), the transient acidosis was abolished and pHi increased rapidly to give a sustained alkalosis (7.49 +/- 0.03 and 7.44 +/- 0.03 respectively). In the presence of 10(-3) mol/l amiloride, the response to acetylcholine was a rapid decrease in pHi to 7.02 +/- 0.02. The data suggest that, during perfusion with HCO3-/CO2- buffered solutions, stimulation with acetylcholine results in a transient loss of HCO3- from the acinar cells (causing a transient acidosis), and, independently, the activation of Na+-H+ exchange (causing a sustained alkalosis). In the unstimulated gland, DIDS and the HCO3- -free perfusate caused decreases in pHi to 7.12 +/- 0.02 and 7.04 +/- 0.01 respectively. In contrast, amiloride had little effect. The relatively high value of pHi maintained by the unstimulated gland is therefore probably not due to Na+-H+ exchange.
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