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  • Title: Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens.
    Author: Erickson BR, Sealy TK, Flietstra T, Morgan L, Kargbo B, Matt-Lebby VE, Gibbons A, Chakrabarti AK, Graziano J, Presser L, Flint M, Bird BH, Brown S, Klena JD, Blau DM, Brault AC, Belser JA, Salzer JS, Schuh AJ, Lo M, Zivcec M, Priestley RA, Pyle M, Goodman C, Bearden S, Amman BR, Basile A, Bergeron É, Bowen MD, Dodd KA, Freeman MM, McMullan LK, Paddock CD, Russell BJ, Sanchez AJ, Towner JS, Wang D, Zemtsova GE, Stoddard RA, Turnsek M, Guerrero LW, Emery SL, Stovall J, Kainulainen MH, Perniciaro JL, Mijatovic-Rustempasic S, Shakirova G, Winter J, Sexton C, Liu F, Slater K, Anderson R, Andersen L, Chiang CF, Tzeng WP, Crowe SJ, Maenner MJ, Spiropoulou CF, Nichol ST, Ströher U.
    Journal: J Infect Dis; 2016 Oct 15; 214(suppl 3):S258-S262. PubMed ID: 27587631.
    Abstract:
    During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its utility in monitoring specimen quality, comparing results with different specimen types, and interpretation of results. For live patients, blood is the most sensitive specimen type and oral swabs have little diagnostic utility. However, swabs are highly sensitive for diagnostic testing of corpses.
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