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  • Title: Identification of Nsp100 as elongation factor 2 (EF-2).
    Author: Koizumi S, Ryazanov A, Hama T, Chen HC, Guroff G.
    Journal: FEBS Lett; 1989 Aug 14; 253(1-2):55-8. PubMed ID: 2759242.
    Abstract:
    The nerve growth factor-sensitive phosphoprotein from PC12 cells, previously designated Nsp100, has been shown to be elongation factor 2 (EF-2). The criteria used for this identification include: (i) similarity of N-terminal sequence; (ii) phosphorylation by the same kinase; (iii) ADP-ribosylation mediated by diphtheria toxin; (iv) comparable function in cell-free protein synthesis. According to these criteria, Nsp100 and EF-2 are identical and the kinase that phosphorylates Nsp100 in PC12 cells is calcium/calmodulin kinase III.
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