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  • Title: Relationships between vitamin E status and erythrocyte stability in sheep.
    Author: Stevenson LM, Jones DG.
    Journal: J Comp Pathol; 1989 May; 100(4):359-68. PubMed ID: 2760270.
    Abstract:
    Stability of erythrocytes from sheep with vitamin E deficiency (E-deficient) was compared by two haemolytic tests, one based on osmotic fragility in hypotonic saline and the other on detergent sensitivity in Tween 20. Relationships between haemolysis, plasma alpha-tocopherol concentration and creatine kinase (CK) activity were evaluated. E-deficient animals were clinically healthy but had elevated (greater than 1000 iu per 1) CK activities and low (less than 1 mumol per 1) alpha-tocopherol concentrations compared with control sheep (less than 500 iu per 1 and greater than 1.5 mumol per 1, respectively). Erythrocytes from E-deficient sheep were markedly more susceptible to detergent treatment than those from controls, but osmotic fragility was similar in both groups. Detergent sensitivity was directly related to plasma alpha-tocopherol concentration and CK activity but not to erythrocyte glutathione peroxidase activity. Within 24 h of supplementation (300 mg alpha-tocopherol subcutaneously) of E-deficient sheep, plasma alpha-tocopherol concentrations were increased, CK activities decreased and erythrocyte susceptibility to detergent-induced haemolysis was significantly reduced. Concentrations of alpha-tocopherol returned rapidly (less than 3 days) to pre-supplement values, although decreased haemolytic responses persisted longer (greater than 7 days). Cells from E-deficient animals were protected against detergent-induced haemolysis by preincubation with alpha-tocopherol in vitro. The results indicate that detergent sensitivity of erythrocytes may provide the basis of a simple functional test for vitamin E deficiency in sheep.
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