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  • Title: Identification and Characterization of SUMO-SIM Interactions.
    Author: Husnjak K, Keiten-Schmitz J, Müller S.
    Journal: Methods Mol Biol; 2016; 1475():79-98. PubMed ID: 27631799.
    Abstract:
    The covalent attachment of SUMO to lysine residues of cellular proteins serves as an important mechanism for the dynamic control of protein networks. SUMO conjugates typically mediate selected protein-protein interactions by binding to specific recognition modules. Identification of SUMO-binding proteins and the characterization of the binding motifs are key to understanding SUMO signaling. Here we describe two complementary approaches that are used to tackle these questions.
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