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  • Title: First detection of Leishmania kDNA in canine cerumen samples by qPCR.
    Author: Belinchón-Lorenzo S, Parejo JC, Iniesta V, Fernández-Cotrina J, Muñoz-Madrid R, Monroy I, Baz V, Gómez-Luque A, Serrano-Aguilera FJ, Barneto JL, Gómez-Nieto LC.
    Journal: Vet Parasitol; 2016 Sep 15; 228():65-68. PubMed ID: 27692333.
    Abstract:
    Nowadays, searching for alternative non-invasive methods for molecular diagnosis of canine visceral leishmaniosis is getting increasingly important. We previously described the presence of Leishmania kinetoplast DNA (kDNA) in canine hair; in this case we hypothesized whether foreign DNA might be present in cerumen of dogs with leishmaniosis, and be detected by Real time quantitative PCR (qPCR). A population of 38 dogs that lived in Leishmania endemic areas was divided in two groups: A (33 dogs with confirmed leishmaniosis by serological techniques) and B (5 healthy dogs). Blood, lymph node, bone marrow and cerumen samples from all animals were tested for the presence of parasite kDNA. Our method was 100% specific, and in dogs from group A, Leishmania infantum kDNA was detected and quantified in the 100% of lymph node samples, in 90.9% of cerumen samples, in 88.5% of the bone marrow samples and in 57.6% of the blood samples. The qPCR-cerumen is a new non-invasive method that shows a high potential for the diagnosis of zoonotic visceral leishmaniosis.
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