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  • Title: The expression, localization, and effect of a human interferon in plants.
    Author: De Zoeten GA, Penswick JR, Horisberger MA, Ahl P, Schultze M, Hohn T.
    Journal: Virology; 1989 Sep; 172(1):213-22. PubMed ID: 2773316.
    Abstract:
    The ORF II of Cauliflower mosaic virus (CaMV) DNA was replaced with the human IFN alpha D coding sequence to yield a stable CaMV strain designated Ca524i. Inoculation of turnip (Brassica rapa cv "Just Right") with strain Ca524i DNA excised from plasmid pCa524i resulted in the production of biologically active IFN alpha D in infected plants. This was also true for its mutant (Ca562i) where one of the CYS codons was deleted. IFN alpha D produced in planta did not hamper superinfection with a single-stranded (+) sense RNA plant virus, turnip yellow mosaic virus (TYMV). Antibody gold labeling techniques and electron microscopy of infected plants showed that IFN was localized in the CaMV inclusion bodies.
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