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  • Title: Establishment of a new teleost brain cell line (DLB-1) from the European sea bass and its use to study metal toxicology.
    Author: Morcillo P, Chaves-Pozo E, Meseguer J, Esteban MÁ, Cuesta A.
    Journal: Toxicol In Vitro; 2017 Feb; 38():91-100. PubMed ID: 27746373.
    Abstract:
    In teleost fish, there are no commercial cell lines for the European sea bass (Dicentrarchus labrax). Thus, we have established the sea bass brain (DLB-1) cell line, using a fish retrovirus for immortalization, which resemble epithelial cells and express glial cells markers. Exposure to metals [Cd, methylmercury (MeHg), Pb or As] produces cytotoxicity and induction of reactive oxygen species (ROS) production. Interestingly, cell cycle analysis of DLB-1 cells shows that exposure to metals alters it significantly. Moreover, all the metals induce apoptosis as indicated by sub-Go/G1 population and annexin V binding. Finally, exposure of DLB-1 cells to metals also produces significant alterations at gene expression level, which confirm the above functional results. This is the first study in which metal cytotoxicity has been evaluated in a fish brain cell line and results seem to support that DLB-1 cells are suitable for toxicological studies.
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