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  • Title: How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot.
    Author: Beyer M, Kiweler N, Mahboobi S, Krämer OH.
    Journal: Methods Mol Biol; 2017; 1510():355-364. PubMed ID: 27761834.
    Abstract:
    Histone deacetylases (HDACs) catalyze the deacetylation of lysine residues in their target proteins. This biochemical modification can have profound effects on the functions of these proteins and a dysregulation of HDAC activity contributes to severe diseases, including neoplastic transformation. In the following chapter, we present a strategy that allows to distinguish between the inhibition of the class I HDACs HDAC1, 2, and 3 and of the class IIb HDAC HDAC6. This method is based on Western blot and relies on the detection of hyperacetylated substrates of class I or class IIb HDACs in lysates from cells that were treated with histone deacetylase inhibitors (HDACi).
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