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  • Title: Estimation of cholesterol loading of the low-density lipoprotein fraction in diabetic subjects without ultracentrifugation.
    Author: Yoshino G, Iwai M, Matsuba K, Iwatani I, Matsushita M, Kazumi T, Baba S.
    Journal: Diabetes Res Clin Pract; 1989 Aug 01; 7(2):155-7. PubMed ID: 2776655.
    Abstract:
    We report here a new formula for estimating apolipoprotein (apo) B concentration in the low-density lipoprotein (LDL) fraction from measurements of plasma triglyceride and apoB. ApoB in plasma and in the triglyceride-rich lipoprotein fraction (VLDL, d less than 1.019) and plasma triglyceride were measured in 112 subjects, including 56 diabetics. There was a significant correlation between VLDL-apoB and plasma triglyceride (Y = 0.07X + 1, r = 0.73, P less than 0.001). We calculated LDL-apoB according to this formula: LDL-apoB = total apoB - (0.07 x total triglyceride + 1). We found an excellent relationship between LDL-apoB (total apoB - VLDL-apoB) and calculated LDL-apoB (Y = 1.0X + 1, r = 0.96, P less than 0.001). This new formula will enable us to estimate the apoB concentration in the LDL fraction without ultracentrifugation.
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