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  • Title: Escherichia coli in marine water: Comparison of methods for the assessment of recreational bathing water samples.
    Author: Lušić DV, Jozić S, Cenov A, Glad M, Bulić M, Lušić D.
    Journal: Mar Pollut Bull; 2016 Dec 15; 113(1-2):438-443. PubMed ID: 27771099.
    Abstract:
    Bathing Water Directive (2006/7/EC) specifies two reference methods for Escherichia coli detection: ISO 9308-1 and 9308-3. The revised ISO 9308-1 is recommended only for waters with a low bacterial background flora. Considering the extended time needed for analysis and, generally, the lack of experience in using ISO 9308-3 in the Mediterranean, the suitability of ISO 9308-1 for the examination of E. coli in bathing water was evaluated. The present study was aimed at a comparison of data obtained by the reference method in seawater samples (110 beaches, N=477) with data received from six alternative methods. Results show that recently used TSA/TBA method may overestimate E. coli numbers in marine waters. The temperature modified ISO 9308-1 (44°C) did not significantly alter the results, but outperformed the antibiotic supplemented agar at reducing non-E. coli bacteria on the plates, allowing the use of the respective method for monitoring coastal water.
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