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  • Title: Different Hoechst 33342 and DAPI fluorescence of the human Y chromosome in bivariate flow karyotypes.
    Author: Bernheim A, Miglierina R.
    Journal: Hum Genet; 1989 Sep; 83(2):189-93. PubMed ID: 2777260.
    Abstract:
    The staining properties of AT-specific dyes Hoechst 33342 and DAPI as revealed by Hoechst 33342/mithramycin and mithramycin/DAPI bivariate human flow karyotype patterns are different for chromosomes rich in heterochromatin. The peak corresponding to chromosome Y of a given cell line is higher on the A/T axis with mithramycin/DAPI staining than with mithramycin/Hoechst. The chromosome 1 was found slightly more fluorescent in mithramycin/DAPI than in mithramycin/Hoechst as judged by the slight displacement of its area on the Hoechst-DAPI axis. The other peaks did not show major differences. On the same flow karyotypes, chromosomal rearrangements were detected.
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