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  • Title: Label/quencher-free detection of single-nucleotide changes in DNA using isothermal amplification and G-quadruplexes.
    Author: Lee IJ, Goo NI, Kim DE.
    Journal: Analyst; 2016 Nov 28; 141(24):6503-6506. PubMed ID: 27827492.
    Abstract:
    We report an analytical method that exploits the interaction between G-quadruplexes and thioflavin T (ThT), for detecting mutant DNA species containing single-base changes. This system is a label/quencher-free fluorescence enhancement system based on rolling circle amplification (RCA)-responsive G-quadruplex formation, which enables a highly selective detection of T790M SNPs in the gene encoding the epidermal growth factor receptor, EGFR.
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