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  • Title: Ultraslow Domain Motions in HIV-1 TAR RNA Revealed by Solid-State Deuterium NMR.
    Author: Huang W, Emani PS, Varani G, Drobny GP.
    Journal: J Phys Chem B; 2017 Jan 12; 121(1):110-117. PubMed ID: 27930881.
    Abstract:
    Intrinsic motions may allow HIV-1 transactivation response (TAR) RNA to change its conformation to form a functional complex with the Tat protein, which is essential for viral replication. Understanding the dynamic properties of TAR necessitates determining motion on the intermediate nanosecond-to-microsecond time scale. To this end, we performed solid-state deuterium NMR line-shape and T1Z relaxation-time experiments to measure intermediate motions for two uridine residues, U40 and U42, within the lower helix of TAR. We infer global motions at rates of ∼105 s-1 in the lower helix, which are much slower than those in the upper helix (∼106 s-1), indicating that the two helical domains reorient independently of one another in the solid-state sample. These results contribute to the aim of fully describing the properties of functional motions in TAR RNA.
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