These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Comparison of proteins and nucleic acids of Echinochloa ragged stunt and rice ragged stunt viruses.
    Author: Chen CC, Hsu YH, Chen MJ, Chiu RJ.
    Journal: Intervirology; 1989; 30(5):278-84. PubMed ID: 2793400.
    Abstract:
    Echinochloa ragged stunt virus (ERSV) and rice ragged stunt virus (RRSV; Reoviridae) were purified from their respective host plants and disrupted in SDS. Evidence for the double-strandedness of ERSV RNA was obtained. Electrophoresis in 10% polyacrylamide gels resolved the RNAs of each virus into 10 segments, ranging for ERSV from 0.61 x 10(6) to 2.8 x 10(6) daltons, with a sum representing the total genome of 17.89 x 10(6) daltons, and for RRSV from 0.6 x 10(6) to 2.98 x 10(6) daltons, with a sum of 18.15 x 10(6) daltons. Although the overall RNA pattern of the two viruses was similar, there were distinctive features. Using 7.5-15% polyacrylamide gels, electrophoresis of SDS-dissociated RRSV particles yielded five major proteins of 129 x 10(3), 123 x 10(3), 63 x 10(3) and 35 x 10(3) daltons, and two minor proteins of 113 x 10(3) and 88 x 10(3) daltons, respectively, while comparable samples of ERSV yielded four major proteins of 127 x 10(3), 123 x 10(3), 63 x 10(3) and 34 x 10(3) daltons, and three minor proteins of 103 x 10(3), 50 x 10(3) and 49 x 10(3) daltons, respectively. We conclude from this and other evidence that ERSV and RRSV are closely related viruses and should be classified in the same subgroup, despite reported differences in the morphology of their outer capsids.
    [Abstract] [Full Text] [Related] [New Search]