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  • Title: Measuring Ca2+ inside intracellular organelles with luminescent and fluorescent aequorin-based sensors.
    Author: Alonso MT, Rojo-Ruiz J, Navas-Navarro P, Rodríguez-Prados M, García-Sancho J.
    Journal: Biochim Biophys Acta Mol Cell Res; 2017 Jun; 1864(6):894-899. PubMed ID: 27939433.
    Abstract:
    GFP-Aequorin Protein (GAP) can be used to measure [Ca2+] inside intracellular organelles, both by luminescence and by fluorescence. The low-affinity variant GAP3 is adequate for ratiometric imaging in the endoplasmic reticulum and Golgi apparatus, and it can be combined with conventional synthetic indicators for simultaneous measurements of cytosolic Ca2+. GAP is bioorthogonal as it does not have mammalian homologues, and it is robust and functionally expressed in transgenic flies and mice, where it can be used for Ca2+ measurements ex vivo and in vivo to explore animal models of health and disease. This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech.
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