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  • Title: [Methodologic recommendations for the determination of 25-OH vitamin D3 in plasma using HPLC].
    Author: Matthey M, Graf H, Richter GH, Flachowsky G.
    Journal: Nahrung; 1989; 33(6):503-7. PubMed ID: 2797115.
    Abstract:
    A method for measuring 25-OH D3-vitamin (calcidiol) in plasma samples of cattle is described. The samples were purified using non-polar C 18-cartridges and 25-OH D3 was determined by high-performance liquid chromatography (HPLC) on a silicic acid straight phase liquid chromatography column (retention time 11.5 min). The detection limit for 25-OH D3 is about 0.5 ng injection quantity, the average recovery was 90%, the standard error amounted to 8%.
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