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  • Title: Deletion of the sclerotome-enriched lncRNA PEAT augments ribosomal protein expression.
    Author: Stafford DA, Dichmann DS, Chang JK, Harland RM.
    Journal: Proc Natl Acad Sci U S A; 2017 Jan 03; 114(1):101-106. PubMed ID: 27986952.
    Abstract:
    To define a complete catalog of the genes that are activated during mouse sclerotome formation, we sequenced RNA from embryonic mouse tissue directed to form sclerotome in culture. In addition to well-known early markers of sclerotome, such as Pax1, Pax9, and the Bapx2/Nkx3-2 homolog Nkx3-1, the long-noncoding RNA PEAT (Pax1 enhancer antisense transcript) was induced in sclerotome-directed samples. Strikingly, PEAT is located just upstream of the Pax1 gene. Using CRISPR/Cas9, we generated a mouse line bearing a complete deletion of the PEAT-transcribed unit. RNA-seq on PEAT mutant embryos showed that loss of PEAT modestly increases bone morphogenetic protein target gene expression and also elevates the expression of a large subset of ribosomal protein mRNAs.
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