These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c.
    Author: Yan YJ, He XW, Li WY, Zhang YK.
    Journal: Biosens Bioelectron; 2017 May 15; 91():253-261. PubMed ID: 28013020.
    Abstract:
    A novel fluorescent sensor nitrogen-doped graphene quantum dots (N-GQDs)/SiO2/molecular imprinting polymer（N-GQDs/SiO2/MIP）was fabricated by surface imprinting and epitope imprinting to recognize and detect the target protein cytochrome c (Cyt C) with fluorescence quenching. In the polymerization process, the C- and N-terminal nonapeptides of Cyt C were selected as the double templates which were fixed by functional monomer (zinc acrylate) through metal chelation and steady six-membered ring. The linear range of fluorescence quenching for this receptor towards Cyt C was 0.20-60μM, and the detection limit was 0.11μM. The precision for six times replicate determination of Cyt C at 30μM was 1.20%, and the imprinting factor (IF) was 3.06. The recoveries of the material to Cyt C in urine were 99.3-114.0%. In brief, this work proposed a strategy to prepare a new type fluorescent imprinting polymer based on N-GQDs and provided an attractive perspective for the detection of protein by using the combination of N-GQDs and molecular imprinting technique.
    [Abstract] [Full Text] [Related] [New Search]