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  • Title: Genoprotective properties of astaxanthin revealed by ionizing radiation exposure in vitro on human peripheral blood lymphocytes.
    Author: Pilinska MA, Кurinnyi DA, Rushkovsky SR, Dybska OB.
    Journal: Probl Radiac Med Radiobiol; 2016 Dec; 21():141-148. PubMed ID: 28027548.
    Abstract:
    OBJECTIVE: to identify possible radioprotective properties of astaxanthin by means of cytogenetic criteria. METHODS: Cultivation of peripheral blood lymphocytes from five apparently healthy volunteers; treatment of lym phocytes' cultures by astaxanthin in final concentrations 20 μg/ml in Go phase of mitotic cycle, prior to ? irradia tion in vitro in a dose 1 Gy; cytogenetic analysis the uniformly stained slides of metaphase chromosomes. The elec trophoresis of individual cells (Comet assay); visualization of results under fluorescent microscope; accounting the number of nucleoid the fourth grade that correspond to apoptosis of the cells. RESULTS: Established that astaxanthin in final concentration 20.0 μg/ml exposed to the culture of human peripher al blood lymphocytes in the early G0 phase of mitotic cycle leads to significant reduction of cytogenetic effects induced by gamma irradiation in vitro in dose 1.0 Gy (from 26.05 ± 1.81 to 9.08 ± 0.78 per 100 cells, respectively) and to significant increase the frequency of apoptotic cells at the 48 hour of cultivation (from (3.78 ± 0.24) to (8.26 ± 0.91) %, respectively). CONCLUSIONS: The results obtained show the ability of astaxanthin to considerable weakening of radioinduced muta genic effect in human peripheral blood lymphocytes, which testify its powerful radioprotective potential. Meta: vyznachyty mozhlyvi radioprotektorni vlastyvosti astaksantynu za tsytogenetychnymy kryteriiamy. Metody. Kul'tyvuvannia limfotsytiv krovi 5 umovno zdorovykh volonteriv; obrobka kul'tur limfotsytiv astaksan tynom v kontsentratsiï 20,0 mkg/ml na G0 stadiï mitotychnogo tsyklu pered gamma oprominenniam kul'tur limfotsytiv in vitro v dozi 1,0 Gr; prygotuvannia ta tsytogenetychnyy̆ analiz rivnomirno zabarvlenykh preparativ metafaznykh khromosom. Metod kometnogo elektroforezu okremykh klityn (Comet assay); vizualizatsiia rezul'tativ pid liumines tsentnym mikroskopom; pidrakhovuvannia kil'kosti nukleoïdiv chetvertogo klasu, iaki vidpovidaiut' apoptychnomu stanu klitnyn.Rezul'taty. Vstanovleno, shcho astaksantyn v kintseviy̆ kontsentratsiï 20,0 mkg/ml pry diï v kul'turi limfotsytiv pe ryferychnoï krovi liudyny na ranniy̆ presyntetychniy̆ (G0) stadiï mitotychnogo tsyklu pryzvodyt' do suttievogo zny zhennia tsytogenetychnogo efektu, indukovanogo gamma oprominenniam in vitro v dozi 1,0 Gr {z 26,05 ± 1,81 do 9,08 ± 0,78 na 100 klityn, vidpovidno} ta znachnogo zrostannia chastoty apoptychnykh klityn na 48 y̆ godyni kul'tyvuvannia {z (3,78 ± 0,24) do (8,26±0,91) %, vidpovidno}.Vysnovky. otrymani rezul'taty pokazaly spromozhnist' astaksantynu do suttievogo oslablennia mutagennoï diï ionizuiuchogo vyprominiuvannia v limfotsytakh peryferychnoï krovi liudyny, shcho svidchyt' pro y̆ogo potuzhnyy̆ radiop rotektornyy̆ potentsial.
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