These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Improved stability of 2,3-bisphosphoglycerate during storage of hexokinase-overloaded erythrocytes.
    Author: Magnani M, Rossi L, Bianchi M, Serafini G, Zocchi E, Laguerre M, Ropars C.
    Journal: Biotechnol Appl Biochem; 1989 Oct; 11(5):439-44. PubMed ID: 2803567.
    Abstract:
    Human red blood cells were overloaded with homogeneous human hexokinase using a procedure of encapsulation based on hypotonic hemolysis and isotonic resealing and reannealing to achieve a final activity that was 15 times higher than that in control cells. Storage for 5 weeks at 4 degrees C of hexokinase-overloaded erythrocytes shows that these cells undergo small K+ leakage and mean cell volume increase compared with control cells. Furthermore, after these 5 weeks of storage the 2,3-bisphosphoglycerate content was normal while the ATP concentration was slightly reduced. These results and other properties suggest that encapsulation of key glycolytic enzymes in erythrocytes can provide a new way to maintain in vitro functionally active red blood cells for at least 5 weeks.
    [Abstract] [Full Text] [Related] [New Search]