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  • Title: A facile label-free G-quadruplex based fluorescent aptasensor method for rapid detection of ATP.
    Author: Liu H, Ma C, Ning F, Chen H, He H, Wang K, Wang J.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2017 Mar 15; 175():164-167. PubMed ID: 28038373.
    Abstract:
    The present work demonstrates a simple, rapid and label-free ATP detection method using a fluorescent aptasensor that is based on G-quadruplex formation. In the absence of ATP, the Thioflavin T (ThT) dye binds to the G-rich ATP aptamer and forms an ATP aptamer/ThT G-quadruplex complex, which results in high fluorescence intensity. Upon addition of ATP, the ATP aptamer/ThT complex will be replaced by the formation of an ATP aptamer/ATP complex. During this process, separation of the ThT dye from the ATP aptamer/ThT complex decreases the fluorescence intensity of the reaction mixture dramatically. This fluorescence aptasensor is highly sensitive and rapid, with a detection limit of 18nM and a total reaction time of only 10min. Furthermore, this method is cost-effective and simple, removing the requirement for labeling the detection reagents with a fluorophore-quencher pair.
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