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  • Title: An excited state intramolecular proton transfer dye based fluorescence turn-on probe for fast detection of thiols and its applications in bioimaging.
    Author: Zhao Y, Xue Y, Li H, Zhu R, Ren Y, Shi Q, Wang S, Guo W.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2017 Mar 15; 175():215-221. PubMed ID: 28040571.
    Abstract:
    In this study, a new fluorescent probe 2-(2'-hydroxy-5'-N-maleimide phenyl)-benzothiazole (probe 1), was designed and synthesized by linking the excited state intramolecular proton transfer (ESIPT) fluorophore to the maleimide group for selective detection of thiols in aqueous solution. The fluorescence of probe 1 is strongly quenched by maleimide group through the photo-induced electron transfer (PET) mechanism, but after reaction with thiol, the fluorescence of ESIPT fluorophore is restored, affording a large Stokes shifts. Upon addition of cysteine (Cys), probe 1 exhibited a fast response time (complete within 30s) and a high signal-to-noise ratio (up to 23-fold). It showed a high selectivity and excellent sensitivity to thiols over other relevant biological species, with a detection limit of 3.78×10-8M (S/N=3). Moreover, the probe was successfully applied to the imaging of thiols in living cells.
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