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  • Title: Evaluation and optimization of variables in immunofixation electrophoresis for the detection of IgG paraproteins.
    Author: Monos DS, Bina M, Kahn SN.
    Journal: Clin Biochem; 1989 Oct; 22(5):369-71. PubMed ID: 2805339.
    Abstract:
    We examined different antisera and varying serum dilutions for their effects on the sensitivity of immunofixation electrophoresis (IFE) for detecting and characterizing serum IgG paraproteins. Different antisera reacted unequally with a single paraprotein and conversely, a single antiserum reacted unequally with several different paraproteins. The dilution of serum was extremely important; the adoption of a single dilution for all sera could result in failure to detect low-concentration or poorly immunoreactive paraproteins. While the application of a standardized amount of paraprotein is recommended, it must be emphasized that no single antiserum can be relied on to provide unequivocal detection of all paraproteins of that particular isotype.
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