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  • Title: A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3'-terminal 2'-O-methylated plant microRNA.
    Author: Wang X, Wang H, Liu C, Wang H, Li Z.
    Journal: Chem Commun (Camb); 2017 Jan 17; 53(6):1124-1127. PubMed ID: 28054059.
    Abstract:
    Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2'-O-methylated at the 3' end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3'-terminal 2'-O-methylated miRNAs.
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