These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Sodium dodecyl sulphate dependent NADPH oxidation: an alternative method for assaying NADPH-oxidase in a cell-free system.
    Author: Sha'ag D.
    Journal: J Biochem Biophys Methods; 1989 Jul; 19(1):121-8. PubMed ID: 2809064.
    Abstract:
    In assaying NADPH-oxidase activities using macrophage-derived cell-free systems, sodium dodecyl sulphate dependent NADPH oxidation rates were found to correlate strongly with superoxide-dismutase inhibitable cytochrome-c reduction rates. Optimum sodium dodecyl sulphate concentration was in the range 95-110 microM. A theoretical function for the activation and deactivation of NADPH-oxidase by sodium dodecyl sulphate is suggested. This function may serve to estimate the real Vmax of the enzyme and the number of detergent molecules required for the full activation of the enzymatic complex.
    [Abstract] [Full Text] [Related] [New Search]