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  • Title: Expert system computer program for troubleshooting the gel-clot Limulus amebocyte lysate (LAL) assay.
    Author: Tsuji K.
    Journal: J Parenter Sci Technol; 1989; 43(5):240-5. PubMed ID: 2809932.
    Abstract:
    This article describes a knowledge-based or expert system computer program that can assist in troubleshooting the gel-clot Limulus Amebocyte Lysate (LAL) method for the assay of endotoxin in parenteral products and in-process materials. The program may also be used to train new personnel and/or to assist in the development of an LAL method for a new product or material. The program includes LAL assay information for 31 parenteral products and materials. The program uses rules-of-thumb and intuition to diagnose 155 possible LAL assay problems and logically reduce them to 37 recommended problem solving processes. The program also provides references to support its recommendation.
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