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  • Title: Simultaneous determination of lactate, pyruvate, and ascorbate in microdialysis samples from rat brain, blood, fat, and muscle using high-performance liquid chromatography.
    Author: Hallström A, Carlsson A, Hillered L, Ungerstedt U.
    Journal: J Pharmacol Methods; 1989 Sep; 22(2):113-24. PubMed ID: 2811388.
    Abstract:
    An isocratic high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of lactate, pyruvate, and ascorbate in tissue and blood, sampled in vivo using the microdialysis technique. The compounds were separated on a silica-based, reversed-phase column or on a resin-based, cation exchange column. The acids were monitored by ultraviolet detection.
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