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  • Title: Selection of a pure cerebellar granule cell culture by kainate treatment.
    Author: Drejer J, Schousboe A.
    Journal: Neurochem Res; 1989 Aug; 14(8):751-4. PubMed ID: 2812251.
    Abstract:
    Chronic exposure of dissociated cerebellar cultures to 50 microM kainate results in a complete loss of [3H]-GABA release which is a marker of GABAergic interneurons. No loss of granule cells was found and the glutamatergic nature of the granule cells appeared unaltered by the kainate treatment, since evoked release of [3H]-D-aspartate was maintained after kainate exposure. Glial cells in such cultures are virtually eliminated by treatment with an antimitotic such as cytarabine. In consequence a pure culture of cerebellar granule cells virtually free of stellate, basket and glial cells may be obtained by a combined chronic treatment of the cultures with kainate and cytarabine.
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