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  • Title: Development of a method for efficient cost-effective screening of Aspergillus niger mutants having increased production of glucoamylase.
    Author: Zhu X, Arman B, Chu J, Wang Y, Zhuang Y.
    Journal: Biotechnol Lett; 2017 May; 39(5):739-744. PubMed ID: 28138854.
    Abstract:
    OBJECTIVES: To develop an efficient cost-effective screening process to improve production of glucoamylase in Aspergillus niger. RESULTS: The cultivation of A. niger was achieved with well-dispersed morphology in 48-deep-well microtiter plates, which increased the throughput of the samples compared to traditional flask cultivation. There was a close negative correlation between glucoamylase and its pH of the fermentation broth. A novel high-throughput analysis method using Methyl Orange was developed. When compared to the conventional analysis method using 4-nitrophenyl α-D-glucopyranoside as substrate, a correlation coefficient of 0.96 by statistical analysis was obtained. CONCLUSION: Using this novel screening method, we acquired a strain with an activity of 2.2 × 103 U ml-1, a 70% higher yield of glucoamylase than its parent strain.
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