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  • Title: Prion Transfection of Yeast.
    Author: Edskes HK, Kryndushkin D, Shewmaker F, Wickner RB.
    Journal: Cold Spring Harb Protoc; 2017 Feb 01; 2017(2):. PubMed ID: 28148849.
    Abstract:
    Transfection of yeast with amyloid filaments, made from recombinant protein or prepared from extracts of cells infected with a prion, has become an important method in characterizing yeast prions. Here, we describe a method for transmission of [URE3] with Ure2p amyloid that is based on a previously published protocol for transfection with Sup35p filaments to make cells [PSI+]. This method may be used for other prions by changing just the amyloid source, host strain, and plating medium.
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