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  • Title: Impact of pH regulation on multicopper oxidase production and swarming motility in the bacterium Proteus hauseri ZMd44.
    Author: Su C, Chen Z, Ng IS.
    Journal: Biotechnol Appl Biochem; 2018 Mar; 65(2):212-219. PubMed ID: 28150465.
    Abstract:
    Proteus hauseri ZMd44, a biodecolorizing bacterium, has been known to produce electricity and multicopper oxidase (Mco-laccase) under copper induction. However, optimization and regulation of production have not been explored. This study is the first attempt to evaluate several parameters on biomass and Mco-laccase production of P. hauseri ZMd44. Through orthogonal experiments with Taguchi's L9, it was found that P. hauseri ZMd44 was sensitive to pH value. The cells grew relatively quickly at pH 7, thus the biomass and Mco-laccase production reached 1.66 g/L and 1043.6 U/L, respectively. Higher pH values also influenced the swarming motility, which is an important characteristic of P. hauseri ZMd44 that affects urinary tract infection. The swarming circle and the diameter of the swarm, represented by the motility velocity, were found to be more controlled after 24 h of growth at pH 6. The swarming ability of P. hauseri was completely inhibited by the addition of 3 mM copper or zinc ions. Therefore, the Mco-laccase and swarming motility could be controlled by regulating pH and ion content.
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