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  • Title: A label-free GR-5DNAzyme sensor for lead ions detection based on nanoporous gold and anionic intercalator.
    Author: Zhou Y, Zhang J, Tang L, Peng B, Zeng G, Luo L, Gao J, Pang Y, Deng Y, Zhang F.
    Journal: Talanta; 2017 Apr 01; 165():274-281. PubMed ID: 28153254.
    Abstract:
    A label-free electrochemical sensor, based on a classic lead ions (Pb2+)-dependent GR-5DNAzyme as the catalytic unit, disodium-anthraquinone-2,6-disulfonate (AQDS) as DNA intercalator, and nanoporous gold (NPG) for signal amplification, was designed for sensitive and selective detection of Pb2+. Firstly, NPG modified electrode surface were employed as a platform for the immobilization of thiolated probe DNA, and then, hybridized with DNAzyme catalytic beacons. The Pb2+-induced catalytic reaction makes the substrate strand break at the cleavage sitGe irreversibly, which disturbs the formation of DNA strands. AQDS served as an indicator that intercalated into the base-pairing regions of DNAzyme, resulting in a strong electrochemical signal. In the presence of Pb2+, the complementary regions were reduced, due to the fracture of the DNA strand, resulting in the release of AQDS. And a decreased current was obtained, corresponding to Pb2+ concentration. Taking advantage of the amplification effect of NPG electrode for increasing the reaction sites of thiol modified capture probe, the proposed electrochemical biosensor could detect Pb2+ quantitatively, in the range of 1-120nM, with a limit of detection as low as 0.02nM, which is much lower than the maximum contamination level for Pb2+ in drinking water defined by the U.S. Environmental Protection Agency. The electrochemical sensor was also used to detect Pb2+ from real water samples, and the results showed excellent agreement with the values determined by inductively coupled plasma mass spectroscopy. This biosensor showed a promising potential for on-site detecting Pb2+ in aqueous environment.
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