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  • Title: Highly sensitive amperometric detection of cardiac troponin I using sandwich aptamers and screen-printed carbon electrodes.
    Author: Jo H, Her J, Lee H, Shim YB, Ban C.
    Journal: Talanta; 2017 Apr 01; 165():442-448. PubMed ID: 28153281.
    Abstract:
    In this study, we developed a sandwich aptamer-based screen-printed carbon electrode (SPCE) using chronoamperometry for the detection of cardiac troponin I (cTnI), one of the promising biomarkers for acute myocardial infarction (AMI). Disposable three-electrode SPCEs were manufactured using a screen printer, and various modifications such as electrodeposition of gold nanoparticles and electropolymerization of conductive polymers were performed. From the bare electrode to the aptamer-immobilized SPCE, all processes were monitored and analyzed via various techniques such as cyclic voltammetry, electrochemical impedance spectroscopy, and X-ray photoelectron spectroscopy. The quantification of cTnI was conducted based on amperometric signals from the catalytic reaction between hydrazine and H2O2. The fabricated aptasensor in a buffer, as well as in a serum-added solution, exhibited great analytical performance with a dynamic range of 1-100 pM (0.024-2.4ng/mL) and a detection limit of 1.0 pM (24pg/mL), which is lower than the existing cutoff values (40-700pg/mL). Furthermore, the developed sensor showed high sensitivity to cTnI over other proteins. It is anticipated that this potable SPCE aptasensor for cTnI will become an innovative diagnostic tool for AMI.
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