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  • Title: DNA Labeling at Electron Microscopy.
    Author: Thelen N, Thiry M.
    Journal: Methods Mol Biol; 2017; 1560():269-276. PubMed ID: 28155161.
    Abstract:
    Here, we describe a method for locating DNA on ultrathin sections. This technique is compatible with all usual fixation and embedding procedures and can be combined with cytochemical methods. Ultrathin sections are incubated in a medium containing terminal deoxynucleotidyl transferase (TdT) and various non-isotopic nucleotide analogs. The labeled nucleotides bound to the surface of ultrathin sections are then visualized by an indirect immunogold labeling technique. This high-resolution method provides a powerful tool for pinpointing the precise location of DNA within biological material, even where DNA is present in very low amounts.
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