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  • Title: Micro-RNA detection based on fluorescence resonance energy transfer of DNA-carbon quantum dots probes.
    Author: Khakbaz F, Mahani M.
    Journal: Anal Biochem; 2017 Apr 15; 523():32-38. PubMed ID: 28159568.
    Abstract:
    Carbon quantum dots have been proposed as an effective platform for miRNA detection. Carbon dots were synthesized by citric acid. The synthesized dots were characterized by dynamic light scattering, UV-Vis spectrophotometry, spectrofluorimetry, transmission electron microscopy and FT-IR spectrophotometry. The fluorescence quantum yield of the synthesized dots was determined using quinine sulfate as the standard. The FAM-labeled single stranded DNA, as sensing element, was adsorbed on dots by π-π interaction. The quenching of the dots fluorescence due to fluorescence resonance energy transfer (FRET) was used for mir 9-1 detection. In the presence of the complementary miRNA, the FRET did not take place and the fluorescence was recovered.
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